Background: One of the most common invasive procedures in medicine is the placement of a peripheral venous line, which is directly within the scope of work of nurses. Although the procedure belongs to common everyday practice, it is associated with a high failure rate. A successful first attempt to set up a peripheral venous line is extremely important, especially in patients who has difficult peripheral venous access, as it ensures a rapid intravenous administration of drugs and fluids, while an unsuccessful attempt to introduce a catheter represents a burden for the patient, staff and healthcare system. For the successful placement of a peripheral venous cannula in the first attempt, it is very important to identify patients in whom peripheral venous access is difficult, so that additional interventions can be organized and carried out in time. Ultrasound-guided peripheral venous cannula placement is an evidence-based intervention that has been shown to improve placement success, particularly in patients with difficult peripheral venous access. Aim: The objective of this research is to determine the characteristics of patients in whom peripheral venous access is difficult to place, and to determine the self-assessment of anesthesiology technicians regarding the need for additional education in the use of ultrasound when setting up a peripheral venous access. Methods: A cross-sectional study was conducted at the Clinical hospital Sisters of Charity in April, May and June 2023. The respondents in the research were anesthesiology technicians in operating rooms. The questionnaire contained the demographic data of the respondents, and clinical data on the patient who had a peripheral venous line installed. Results: Research on 410 patients showed that 31% of patients belong to the group with a moderate or high risk for setting up a peripheral venous route according to the A-DIVA scale. The results of the research also show that among 41 anesthesiology technicians there is a significant diversity in the perception of the need for additional education in order to improve the success of the placement of the peripheral venous line. Conclusion: The research provides important insights into the factors that influence the placement of peripheral venous access and indicates the need for aditional education and targeted practice to ensure the success of this procedure and optimal care for patients.