Introduction: Hemodialysis is one of three therapeutic methods for replacing the renal function and is actually a method of removing waste products such as potassium and urea, creatinine, as well as water from the blood in case of kidney failure. The other two are kidney transplantation and peritoneal dialysis. Of all therapies, hemodialysis is the most common form of treatment for patients with terminal phase of kidney failure. Patients use dialysis three times a week, and the procedure takes an average of four hours. Objectives: The aim is to determine whether hemodialysis affects the quality of patients life and if there are any differences in the personal assessment of the life quality of patients on hemodialysis regarding to age, sex , number of years that they are attending the dialysis, and the dialysis approach. Methods: The study was proven among 95 patients at the "Klinička bolnica Merkur" in the Department of Nephrology, Transplant Clinic, and Department of Renal Replacement Treatment. An anonymous questionnaire was used that was developed for the purpose of this research. Prior to the research, the approval of the Ethics Committee of KB Merkur was obtained. Data was analyzed by statistical methods. Results: The results of the research show that patients treated with hemodialysis have the support of the people around them (family and friends), they pay attention to nutrition and strive to eat healthy, they are satisfied with hospital services and believe that kidney transplantation would greatly improve their quality of life. Analysis of the obtained data shows the difference between the age groups. Younger people feel that they do not enjoy life like other people, the fistula affects their appearance, while older people have other difficulties such as going on hemodialysis and they have a problem keeping an eye on fluid intake. Women are more likely to take pain therapy and find that chronic illnesses make their lives even more difficult. Patients which are on dialysis for a long time feel that they cannot live alone and feel that they are taking too much medication. Conclusion: The obtained results increase awareness of an extremely important segment of care for dialysis patients, because their quality of life is a measure of treatment outcomes.