Introduction: Antibiotics have revolutionized treatment of common bacterial infections and have played a vital role in reducing mortality. Since the introduction of antibiotics, their consumption has increased dramatically in most parts of the world. However, inappropriate use of antibiotics is one of the long-standing problem in the world. Recent research shows that the knowledge of the general public about the proper use of antibiotics is limited. This contributes to the problem of inappropriate use of antibiotics, leading to a progressive loss of bacterial susceptibility to these drugs and the spread of resistant bacterial strains, with significant clinical and economic impact. Aim of the research: Aim of the research is to investigate and determine the differences of the knowledge of students of nursing and students of other non-medical studies of the Croatian Catholic University about the adequate use of antibiotics. Methods: The research tool used in this cross-sectional study was a validated questionnaire “Knowledge, attitudes and practice with respect when using antibiotics” developed by Mallah N, Rodríguez-Cano R, Figueiras A et al. The questionnaire was adapted for the online survey using the Google form, and students have received a link to access the survey via official email. Results: Based on the analysis conducted on a sample of 343 students, it was concluded that nursing students are indeed more educated in the adequate use of antibiotics compared to students of other non-medical studies. The difference in answers was also noticed among students from the families includes the members with medical education, and their level of knowledge is 4.63 points higher compared to students who do not have such kind of family member. The survey showed that older students aged 41 and over showed the highest level of antimicrobial resistance knowledge and good profilactic habits. The analysis of the data did not reveal a difference in students' knowledge with regard to the frequency of antibiotic use in the past year. Conclusion: Most of the students have had a relatively good global knowledge of antibiotics and their use, however, there is always room for improvement. Existing education programs on antibiotic use in secondary schools or colleges need to be enriched with special emphasis on stopping antimicrobial resistance, especially in biomedical studies whose students should be a model of behaviour for citizens and patients.