Background: Protective equipment, from the first gloves to N95 masks and new waterproof coats, has become a routine part of work in Western healthcare facilities. It is easy to assume that what is common is also simple and this assumption has been extended to the use of personal protective equipment. In a hospital setting, it is common to assume that healthcare professionals are skilled in all aspects of personal protective equipment. Aim: The aim of this research is to examine the knowledge and attitudes of undergraduate and graduate students about personal protective equipment and standard protection measures in everyday work. Subjects and methods: A total of 201 students of all ages participated in the study. The research included respondents who are currently studying at the undergraduate and graduate level of nursing at the Croatian Catholic University and the Faculty of Dental Medicine and Health in Osijek. Data were collected through an anonymous questionnaire developed for the purpose of this research only. The obtained data were processed through descriptive statistics shown in graphs, histograms and descriptive. Data were processed via Microsoft Excel and Google Forms for data processing. Results: A total of 201 respondents participated in the study, of which 164 were female (81.6%) and 37 were male (18.4%). At the age of the respondents, it can be noticed that 51.7% (104) of the respondents are aged 19-25, 28.9% of the respondents (58) are aged 26-35, 16.4% of the respondents (33) are aged 36 - 45 years and 3% of respondents (6) are aged 46 - 55 years. A higher percentage shows that graduate students had greater knowledge and better clinical practice compared to undergraduate students. Conclusion: The obtained data confirmed the hypotheses. Strict adherence to the use of personal protective equipment among healthcare professionals is key to preventing exposure to blood-borne infections and the transmission of infections in the workplace; however, breaches of security protocols are not uncommon due to work overload and labor shortages. The COVID-19 pandemic has had a major impact on the perception of the importance of personal protective equipment, as well as compliance with its use, in an effort to alleviate the transmission of infection.