INTRODUCTION Understanding, information and awareness of infections have become crucial in preserving health and suppressing the spread of diseases that significantly affect public health and the quality of life of people around the world. Research based on the assessment of the knowledge and attitudes of health professionals in the future will become an indispensable tool for ensuring a high level of expertise and quality in the competencies of providing care, prevention and education of patients, and above all in their own responsibility towards themselves. AIM To examine the knowledge and attitudes of undergraduate and graduate nursing students at the Croatian Catholic University in Zagreb regarding HBV, HCV, and HIV infections. METHODS A cross-sectional study was conducted among nursing students at the Croatian Catholic University in Zagreb. The research was carried out during June and July 2023 using an online survey questionnaire via LimeSurvey. A convenience sample consisted of a total of 90 students who participated in the study by responding to the link provided by the University's Student Services to the email addresses of nursing students across all years. The anonymous survey questionnaire comprised socio-demographic questions, questions assessing knowledge of HBV, HCV, and HIV infections, and students' attitudes towards the mentioned infections. RESULTS Differences in attitudes towards HBV, HCV and HIV infection showed that students over 25 years of age have a greater understanding of these infections and a greater sense of security in future work with these patients, and their attitude could be characterized as more positive. There is no statistically significant difference in knowledge between undergraduate and graduate nursing students about HBV, HCV and HIV infections. Also, no statistically significant difference between knowledge about HBV, HCV and HIV infections between full-time and part-time nursing students was proven. CONCLUSION Through this research, important factors, such as knowledge and the role of work experience, in the perception and reaction of nursing students to HBV, HCV and HIV infections were highlighted.