Introduction: Health care is characterized by multiple interactions between different participants, which often call into question the preservation of professional secrecy. Such situations emphasize the role and importance of continuous education about professional confidentiality as an essential element in the daily work of a nurse/technician. Aim: The aim of this research is to examine the attitudes and knowledge nursing students at the Croatian Catholic University employed in the healthcare system regarding the preservation of professional confidentiality. Methods: A cross-sectional study was conducted among nursing students at the Croatian Catholic University employed in the healthcare system. Anonymous questionnaire consisting of three parts was used to collect data from the students. The research was conducted on the premises of the Croatian Catholic University. The first part of the questionnaire consisted of 9 questions related to sociodemographic data, the second part consisted of 14 questions regarding students’ attitudes towards professional confidentiality, and the third part consisted of 7 questions regarding students’ knowledge about professional confidentiality. The sociodemographic questions in the questionnaire were tailored to the needs of this thesis, while the questions about the participants’ attitudes and knowledge about professional confidentiality were adapted from a scientific paper by a group of authors (literature reference: Tegegne MD. et al. Health professionals’ knowledge and attitude towards patient confidentiality and associated factors in a resource-limited settings: a cross-sectional study. BMC Med Ethics. 2022;23(26):1-10). This survey is validated, reliable and did not require author's permission to use it. Results: A total of 170 nursing students employed in the healthcare system participated in the study. Among them, 145 (85,3%) were females, 45 (26,5%) the half in the undergraduate program, 85 (50%) mainly between 25 and 40 years old. The one hundred and six were unmarried (51,8%) and 89 had 5 years or less of work experience (52,4%). They mainly received education in medical ethics (153; 90%). (157), received had a higher vocational education degree, 153 (90%) had received education in medical ethics, 114 (67,1%) claimed not to disclose access data, 93 (54,7%) while almost a half of them (48,2%) experienced more than two moral dilemmas (48,2%) and had 21 or more patients per day. Conclusion: The results indicate that students at Croatian Catholic University possess a high level of knowledge regarding professional confidentiality and positive attitudes. However, the negative attitudes towards professional confidentiality (30%) suggest that there is room for improvement and that greater emphasis should be placed on this area.