Background: Hypoglycemia is one of the acute complications of diabetes associated with glucose-lowering therapies such as insulin or sulfonylurea. Hypoglycemia is defined as a low blood glucose level (below 4 mmol/L), and it can be classified as mild, severe, or relative hypoglycemia. Nurses are members of a multidisciplinary team who, along with doctors and other staff, participate in patient care and therefore must possess specific knowledge and skills to better recognize and manage hypoglycemic episodes. This study examines the knowledge and attitudes of nurses/technicians in the Republic of Croatia. Since no research on this topic has been conducted in the Republic of Croatia so far, and the number of people with diabetes is increasing, it is essential for nurses/technicians to know how to respond promptly and manage a hypoglycemic episode as an emergency situation. Aim: The aim of this research is to examine the attitudes and knowledge of nurses about the management of hypoglycemia in the Republic of Croatia and whether there is a difference with regard to the level of education and work experience. Methods: An observational cross-sectional study was conducted, and the respondents were nurses/technicians in the Republic of Croatia. The data was collected through an online survey that was adapted for the needs of this thesis. The research was conducted from November to December 2024. Data were collected using the survey questionnaire "Questionnaire for nursing staff on inpatient hypoglycaemic (low blood glucose) management," which was taken from the article published by C. Jones and J. Ndebu, and the questionnaire from the article "Knowledge, Attitudes and Practices of Health Care Providers in the Philippine General Hospital towards In-Patient Hypoglycemia and its Management," published by Isnani S-LJ, Macalalad-Josue A, and Jimeno CA. The questionnaires were adapted for the purposes of this thesis. Results:The study included 317 respondents, of which 256 (80.76%) were female and 61 (19.24%) were male. The results indicate that a significant number of respondents have satisfactory knowledge in the area of hypoglycemia management procedures (89.91% of respondents have at least 50% correct answers), while the majority of respondents have favorable attitudes towards hypoglycemia management (IQR=3.67). The attitudes of nurses/technicians towards hypoglycemia management were assessed using 6 questions, and on a scoring scale ranging from 1 to 5 points, the median attitude score of nurses/technicians towards hypoglycemia management was 3.67 (IQR=2.83-3.83). Among the respondents with bachelor's degrees, the majority achieved a score of more than 50% on the knowledge assessment questionnaire (n=144; 99.31%), with only one respondent scoring less than 50%. The examination established that a statistically significant majority of nurses/technicians scored more than 50% (χ2=141.03; P<0.001). Conclusion: It has been found that nurses/technicians in Croatia possess very good knowledge of hypoglycemia management, excelling particularly in situational questions rather than theoretical ones. Additionally, it has been determined that bachelor's and master's degree holders in nursing exhibit a more positive attitude towards hypoglycemia management compared to nurses with a secondary level of education. Data analysis suggests that nurses/technicians in Croatia have more pronounced clinical knowledge and skills compared to theoretical knowledge, which gives us insight into the need to change the education of this staff.