Background: Physical activity significantly improves the quality of life of adolescents. Technological progress and the modernization of society have increased the time that adolescents spend in front of digital screens. Regular physical activity has a positive effect on physical health, mental well-being, social integration, self-esteem and obesity prevention. Due to their health orientation, the students of the Vinogradska Nursing School have a lot of information about the importance of physical activity and should use their personal example to promote its positive impact on the quality of life. Aim: To examine the relationship between the level of physical activity and the quality of life of students in the third, fourth and fifth grades of the Vinogradska Nursing School. Methods: A cross-sectional study was conducted at the Vinogradska nursing school from May 1 to 15, 2024, in which students of the third, fourth and fifth grades participated. For the purposes of the research, standardized instruments were used: a shorter version of the WHOQWOL-BREF questionnaire, which measures quality of life, and a shorter version of the International Physical Activity Questionnaire (IPAQ). Results: 291 students participated in the research. There is a difference in the assessment of psychological health regarding the gender of the respondent (t=2.2; p=.029); male students rate their mental health better than female students. There is a difference in the frequency of performing extremely strenuous physical activities regarding the gender of the respondents (U=5.374.500; p=.000); male students perform extremely strenuous physical activities more often than female students. Respondents were the least satisfied with their sleep (M=2.9; SD=1.1). They achieve the lowest result in the dimension "mental health" (M=3.6; SD=0.7), which means that they have the lowest quality of mental health. There is a statistical correlation between the assessment of the general quality of life and the frequency of extremely strenuous physical activity (r=.175; p=.003) and moderate physical activity (r=.127; p=.030); with the increase in the frequency of these activities, the general quality of life of the respondents increases. There is a statistically significant negative association between general satisfaction with one's health and the frequency of sitting; with an increase in time spent in a sitting position, there is a decrease in satisfaction with physical health (r=-.157; p=.018). Also, there is a connection between environmental assessment and the frequency of extremely strenuous physical activity (r=.126; p=.031); with the increase in the frequency of these activities, there is a more positive assessment of one's own environment in the applied questionnaire. Conclusion: This research confirms that there is a significant connection between the level of physical activity and the quality of life of students.