Introduction: HPV vaccination is safe and reliable way to protect young people from a number of cancers and diseases related to the HPV virus. In most people, the virus does not cause symptoms and dissapears on its own and sometimes it can remain on the women genital mucosa for a long time and cause malignant changes and cancer. Vaccination includes receiving two doses six months apart in order to provide the best protection against the HPV virus. In Croatia, HPV vaccine is used since 2007, and so far no serious side effects have been recorded. HPV vaccination of the adolescent population is still not significantly high. Aim: To asses the views of final-year high school students in the Šibenik-Knin County regarding HPV vaccination, the HPV virus vaccination rate, general knowledge about the HPV virus and the consequences of HPV infection. Methods: A cross-sectional study was conducted on final-year high school students in the, Šibenik-Knin County area, and the anonymous survey questionnaire consisted ofsociodemographic questions, questions about sexuality, and questions examining views, knowledge, and HPV virus vaccination rates. The study included the Medical School Šibenik, Antun Vrančić Gymnasium, and Ivan Meštrović High School Drniš. In the research, Antun Vrančić Gymnasium in Šibenik and Ivan Meštrović Secondary School in Drniš were grouped under the name „General Gymnasium“ due to the smaller number of students in those schools and the same educational focus. The research was conducted during December 2023, January and February 2024 via an online survey questionnaire using Google Forms. The convenient sample consisted of a total of 109 students. Students accessed the survey through a link, which was forwarded by class teachers to the class of students. Results: In the conducted research, it was proven that there is a statistically significant difference in the knowledge of students from the Medical school Šibenik compared to the students from Antun Vrančić Gymnasium and the students from Ivan Meštrović Secondary School in Drniš. Furthermore, it was not proven that there is a statistically significant difference in the HPV vaccination rates among the three schools. Conclusion: This research, as the first conducted study on HPV virus and HPV vaccination rates in the Šibenik-Knin County, provides a clearer insight into the knowledge and awareness of high school students regarding this public health issue.