Every employee can experience their work differently. The meaning that is attached to work is called work orientation. There are three work orientations: job orientation as a source of income, career orientation, and calling orientation. According to previous research, employees with calling orientation are more satisfied with their work and life, but their quality of life is unknown. That is why the aim of this research was to investigate the relationship between work orientation, job satisfaction and quality of life. As a means of measuring this relationship, Work-life Questionnaire (Wrzesniewski et al., 1997), Index of Job Satisfaction (Brayfield and Rothe, 1951), and World Health Organization Quality of Life Questionnaire (WHOQOL-BREF; WHO, 1996) were used. Research involved 162 employees. The results of hierarchical regression analysis have shown that job orientation as a source of income is a statistically significant and negative predictor of job satisfaction, while calling orientation is a statistically significant and positive job satisfaction predictor. Career orientation is not a statistically significant predictor of job satisfaction. Job orientation is a statistically significant, but weak predictor for the domain of mental and physical health, while it is not a statistically significant predictor for the domains of social relations and the environment. Career orientation and calling orientation are not statistically significant predictors for any domain of quality of life.