Introduction: Given its incidence, suicide is a complex public health problem, not only in Croatia but throughout the world. The fact of such an understanding of suicide as a public health problem stems from WHO data, where in 1998 suicide was the twelfth leading cause of death in the world, and in 2007 suicide was one of the top three causes of death in most countries. There is considered to be no effective algorithm for predicting suicide in clinical practice, but a good recognition and understanding of clinical, psychological, sociological, and biological factors can enable us to identify high-risk individuals and help select appropriate treatment. Suicidal individuals with their thoughts, reflections, and intentions to end their lives require strict supervision by health professionals for twenty-four hours. Objective: The aim of the research is to examine the knowledge of nurses and technicians in the issue of suicide, and to examine whether there is a difference in knowledge of suicide in the population of nurses and technicians depending on the level of education and work experience. Methods: The study included 142 nurses and technicians from the Vrapče University Psychitaric Hospital and the Department of Psychiatry, Sestre Milosrdnice University Hospital Centre. Respondents are nurses and technicians of secondary and higher education, aged 18 to 65 years. The condition for participation in the research was getting acquainted with the purpose of the research and signing an informed consent. Results: The results obtained from the research indicate that there is no statistically significant difference in the knowledge of the issue of suicide of nurses and technicians with regard to education and years of work experience. Conclusion: The problem of suicidal behaviour is a complex public health problem. Further understanding of suicide requires further education of nurses and technicians through all forms of professional development.