Introduction: The importance of laboratory tests is crucial for diagnosis or effective treatment algorithms. The timely and accurate reporting of results has direct impact on patient care. Fulfillment of the established criterias and continuous improvement of the pre-analytical quality indicators depends on healthcare personnel´s compliance to sampling rules. Objective: Employees of Emergency Hospital Admission Ward (EHAW) from University Hospital Center Sestre milosrdnice, Zagreb, Croatia were assesed by using a standardised questionnaire and observation of rutine work processes with the aim to evaluate the staff commitment to recognised venous blood sampling international guidelines. Analysis of the collected data will enable the identification of the key steps for improvement and training will be carried out according to accepted guidelines. The effectiveness of education in lowering serum or plasma hemolysis frequency as well as the clots in samples with anticoagulants will be evaluated. Methods: Fifteen volunteers out of 62 employees were assessed with the use of standardized questionare, 32 were observed during collection of venous blood samplings for the total of one hundred patients and 49 attended the training focused on the identified key steps for improvement of quality indicators. The evaluation of the educational effectiveness was carried out by comparing quality indicators data collected from the laboratory information system (LIS) during a period of thirty days before and after the education. Results: Standardized questionnaires and monitoring of sampling procedures revealed all critical areas for improvements by use of educational tools. The frequency of hemolysis in serum and plasma expressed as a sigma value did not meet the minimum acceptance criteria either before or after the education. The sigma value for the proportion of clotted samples for hematological tests met the minimum criterion of acceptability before and after the education, for coagulation tests the minimum criterion was not met. Conclusion: One-time standardized education did not meet the expected goals in the improvement of pre-analytical quality indicators. Implementation of standardized initial education, continuous reporting and reviewing and periodic repeating of education is necessary for continuum quality indicators improvement.