Background: Occupational exposure, i.e. exposure incident, is contact with potentially contaminated material that may contain blood-borne pathogens and that occurs during professional activities, either inside or outside a health care facility, either in direct work with a patient or in contact with the patient's body fluids and tissues. Aim: The aim of the research was to determine the difference in the number of exposure incidents among healthcare and non-healthcare workers in the Dubrava Clinical Hospital (KBD) in the period before the COVID-19 pandemic compared to the period during the COVID-19 pandemic. Methods: A comparison was made of the number of exposure incidents: a) between the period before the pandemic and the period after the beginning of the pandemic, b) according to the total number of hospitalized patients (expressed as the total number of exposure incidents per 1000 patients per month) for the period before the pandemic and the period during the pandemic and c) according to the total number of active hospital activities (expressed as the number of exposure incidents per 1000 activities per month). Results: In the analyzed period before the COVID-19 pandemic (from March 11, 2018 to March 10, 2020) and during the COVID-19 pandemic (from March 11, 2020 to March 11, 2022) it was 241 exposure incidents reported in KBD. 128 exposure incidents were reported before the pandemic and compared to 113 exposure incidents during the pandemic. Before the pandemic, 90.63% of exposure incidents were recorded in healthcare workers, while during the pandemic 95.58% of exposure incidents were recorded in healthcare workers. The number of exposure incidents in relation to the total number of hospitalized patients was higher during the pandemic (3.93/1000) than in the period before the pandemic (2.50/1000). The number of exposure incidents in relation to the total number of active hospital activities was higher during the pandemic (0.38/1000) than in the period before the pandemic (0.17/1000). Conclusion: The number of exposure incidents according to the total number of hospitalized patients and the total number of active hospital activities in KBD was higher during the pandemic, and the number of total exposure incidents increased during the COVID-19 pandemic among healthcare workers.