It is known that people nowadays lead a sedentary lifestyle, due to the fast pace of life and various technology. Inactivity causes a range of health issues, from cardiovascular and heart diseases, obesity, to serious mental health problems and diseases (Bartoš, 2015). Regular physical activity strengthens the body, develops motor skills, contributes to socialization, improves overall health, positively affects mental health, and reduces the risk of premature death (Bungić and Barić, 2009). The aim of this research is to explore the relationship between personality traits, motivation, and persistence in exercise, thereby contributing to a better understanding of these concepts and realizing the importance and contribution of physical activity to overall human health. The results of this research showed that individuals with higher scores on the dimensions of Extraversion, Conscientiousness, Emotional Stability, and Intellect have a higher motivation to exercise, while Agreeableness was not statistically associated with exercise motivation, which confirmed expectations. As for the relationship between persistence in exercise and personality traits, only Agreeableness proved to be negatively significant, implying that people with higher scores in Agreeableness exercise less, but these results were not in line with the set expectations. A positive correlation was also found between motivation to exercise and persistence in exercise, confirming the expectation that individuals with higher exercise motivation will train more. Through this research, we gained valuable insights into how personality traits can shape our motives and habits related to physical activity. The importance of an individual approach when designing exercise programs is emphasized, considering different personality traits and motivation. In conclusion, these findings may serve as an incentive for further exploration of complex human behavior in the context of health and well-being.