Introduction: The level of knowledge and awareness of students about breast cancer is an important area of research in health education. Understanding their awareness can provide valuable insights into their understanding of risk factors, symptoms and preventive measures related to breast cancer. By analyzing their knowledge, targeted educational interventions can be developed to improve awareness of the disease, as well as to promote early detection strategies and rapid activity. Aim: To assess the knowledge and awareness of male and female students at the Croatian Catholic University regarding breast cancer. Methods: The type of study that was conducted is a cross-sectional study. The selected participants were students of the Croatian Catholic University and the research was conducted using an anonymous online questionnaire, whose access link was distributed via the official email address, in the course of 1st to 31st of March 2023. All of the participants were volunteers and had the option to withdraw from the study at any given time. The questionnaire consisted of sociodemographic questions and questions about the level of knowledge and awareness of breast cancer. Results: The sample consisted of 312 participants, including 122 nursing students, 78 psychology students, 16 history students, 5 medical students, 45 communication studies students, and 46 sociology students. The participants had significantly higher accuracy than 50 % in their responses to breast cancer questions. The results indicate that medical and nursing students answered given questions with statistically notable higher accuracy compared to students from other study programs. Conclusion: The study found that medical and nursing students have a higher level of knowledge and awareness compared to students in social sciences. There is am emphasized need for further education on breast cancer among the student population.