Introduction: Shift work in the field of healthcare represents a form of work organization according to which the individual working hours of each worker ensure the continuous operation of the healthcare institution. This way of working is associated with the development of various health conditions that can impair the quality of life and consequently lead to a reduction in the quality of care provided. Objective: To examine the impact of shift work on the quality of life of nurses and technicians employed at the Vrapče Psychiatry Clinic. Methods: a cross-sectional survey was conducted that included 86 respondents. The research is anonymous and voluntary. Before participating, the subjects were informed about the research and signed a voluntary consent to participate. The research was conducted in 2024. A sociodemographic questionnaire and the SF-36 Health Questionnaire were used. The research was approved by the Ethics Committee of the Vrapče Psychiatry Clinic. Results: It was shown that shift work does not have a significant impact on the quality of life of nurses and technicians in the psychiatry department, and that there are no significant differences in the level of anxiety and the frequency of physical and mental exhaustion in relation to whether the respondents work in shifts or not. The research hypotheses were not confirmed by the obtained results. Conclusion: Regardless of the fact that no connection between shift work and quality of life has been established, the research has shown that the lowest quality of life is shown by respondents with a high school diploma, who all work night shifts. It is recommended to carry out additional research in order to more precisely determine the connection between shift work and factors that have an impact on reducing the quality of life.