Nursing change of shift report or handover can be conducted in different ways. Regardless of the method used, this practice is considered critical to ensure the continuity and quality of healthcare. The purpose of this study was to examine the perception of correlations between nursing handover and the quality of healthcare among the medical nurses/technicians in the “Dr. Josip Bencevic” General Hospital. A total of 109 medical nurses/technicians employed in the departments of gynecology, oncology and hematology, pediatrics, surgery, reanimation, psychiatry and ophthalmology in the “Dr. Josip Bencevic” General Hospital participated in the study. Research was conducted by means of an anonymous questionnaire containing 26 questions with multiple-choice answers. Research results have shown that the most commonly used method of nursing handover is verbal handover, conducted next to the patient’s bed and utilizing nursing documentation, as confirmed by 58 (53.2%) of the survey participants (Chi-square test, P<0.001). Verbal handover combined with nursing documentation, without the presence of patients being is used by 45 (41.3%) of the participants. Eighty (74.1%) of the participants confirmed that the nursing handover documentation contained all the necessary information required to continue providing patient care seamlessly and safely. Significantly more participants feel that healthcare quality is most affected by the information received during nursing handovers, communication within the team and number of available personnel. Furthermore, significantly more participants have general perception that the quality of nursing handover is affected by the skill set and professionalism of the nurses/technicians. 74.1% of the participants feel that it is not necessary to change anything regarding the current practice of nursing handovers (Chi-square test, P<0.001). Results obtained through this study reveal an initial overview of the current nursing handover practices in the “Dr. Josip Bencevic” General Hospital. They can serve as a useful encouragement for further research, as well as to improve and enhance nursing handover documentation which evidently plays a crucial role in the quality of healthcare.