Objective: To determine the relationship of the most common stressors in the work environment with the quality of life of nurses. Materials and methods: The survey was conducted using an anonymous questionnaire among nurses of University hospital Centre Zagreb. The survey analyzed the most common stressors, strategies of coping with stressful situations and quality of life. Respondents estimated their quality of life using an short version of the Quality-of-Life Questionnaire and the Stress Questionnaire. Results: A total of 127 respondents of an average age of 35.5 ± 8.6 was included in the study. In the domains of the quality of life, respondents achieved high levels of a scale maximum of 71 to 79%. The mean rank of measured stress for women was 81.6 and for men 39.42 (P <0.001). The total score of coping with stress (%) was 70.91 for women and 54.36 for men (P = 0.012). A statistically significant (P <0.001) higher score was recorded for men in all domains of the quality of life. Considering how the working hours are organized, work in shifts "12-24-12-48" and two shifts (morning, noon) have a statistically significant protective effect. Conclusion: There is a significant share of respondents with an extremely high level of stress among the nurses, who also have a low level of coping with stress. The perception of the respondents' own health and quality of life is satisfactory, where male respondents achieve higher scores in all domains, especially in coping with stress. There is a significant correlation between duration of internship and total stress, as well as marked differences in stress, coping with stress, and quality of life between men and women.