Introduction: This master’s thesis aims to define the concept of vaccination, present all the mandatory vaccines under the law of the Republic of Croatia, and point out the differences in attitudes about vaccination among students of the Croatian Catholic University. Vaccination against infectious diseases is an extremely important part of public health because it allows us to control certain infectious diseases centuries back to the present day. In the past few decades certain pathologies of a disease have been connected to vaccination, resulting in a major public health problem. Aim of the research: To investigate the different attitudes about vaccination among nursing students, and students with other directions of university. Methods: The research is organized as a cross-sectional study. For the purposes of the research, a validated “Vaccination Attitudes Test Scale” (VAX Scale) by L.R. Martin, and K.J. Petrie. was used. Before accessing the survey, each student received an informed consent in the text of the email. The survey is formulated in three parts, the first part are sociodemographic questions that will examine gender and level of education, the second part consists of 7 questions about behavior and intention about vaccination, and the third part contains 12 questions about examining attitudes about vaccination that are constructed based on the Likert scale from 1 to 5. Data analysis was done in SPSS statistical software. Results: The research was conducted on a sample of 227 respondents. Students of the Croatian Catholic University have proven to be respondents that have positive attitudes about vaccination. Most respondents showed suspicion about vaccination, but the vast majority still believes in vaccination programs. Conclusion: Greater dissemination of useful information about vaccination and about vaccination through social networks and better education of the population could contribute to positive attitudes about vaccination and increase vaccination coverage in the Republic of Croatia.