Background: Endobronchial ultrasound with transbronchial puncture(EBUS-TBNA) is a minimally invasive diagnostic test that helps us diagnose lung diseases, mediastinum, and paratracheal and peribronchial lesions accessible from the respiratory tract. Performing the test is impossible without sedation of the patient. The depth of sedation and securing the airway during EBUS-TBNA can affect the quality of sampling and the frequency of procedure complications. Aim: The objective of the study is to determine the outcomes of EBUS-TBNA depending on the presence of the anesthesiology team. Methods: The medical documentation of the hospital IT system of the Dubrava Clinical Hospital was processed through retrospective research. Data for patients who underwent EBUS-TBNA in the period from January 1, 2020 to October 1, 2022 were analyzed. Results:The study included 70 patients who underwent EBUS-TBNA with the presence of an anesthesiology team (AT) and 70 patients who underwent the same procedure without the presence of AT with sedation and spontaneous breathing under the guidance of a pulmonologist. Data analysis revealed that there was no significant difference in the number of punctures during the examination in the two investigated groups. The total number of punctures with the presence of AT was 357, and in the group without AT 361. Desaturation occurred in 4 (2.9%) patients who were examined with the presence of AT, while in the group of patients who were examined under the guidance of a pulmonologist, it did not occur desaturation. The frequency of bleeding was significantly higher in the group in which the examination was performed without the presence of AT (26 (37.1%) versus 4 (5.7%) patients). Conclusion:The frequency of bleeding during the examination was significantly higher in patients who were examined without the presence of AT, while desaturation occurred only in the group of patients who were examined with AT. Therefore, we can say that the presence of AT during EBUS-TBNA certainly enables the pulmonologist to better focus on conducting the examination with fewer punctures, and also reduces the possibility of bleeding, while the presence or absence of AT does not affect the incidence of desaturation during the examination.