Background: Motivation is based on various human needs, desires and values that differ between each individual, they are changing, developing and directing in proportiom to the growth and development of the individual. Motivation is divided into intrinsic and extrinsic motivation. There are several theories that try to explain motivation, and they are based on different assumptions and emphasize different dimesions of behavior. Work motivation is considered very important in the success and development of the organization because it increases the productivity and efficiency of employees. Aim: The aim of this research is to examine the level of motivation of nurses and technicians at the workplace and to examine the difference in motivation at the workplace in relation to the level of professional education, gender, age, work experience and marital status. Methods: The research was conducted in April 2023. The survey part of the questionnaire was conducted trough the Limesurvey platform and was distributed trough e-mail and social networks. For the purposes of writing the thesis, a cross-sectional study was conducted. Results: 186 respodents were analyzed in the research. Data analysis revealed that there is no significant difference in motivation with regard to the level of education. Job security and good working conditions are important factors that influence the level of motivation. Although the respondents state that they have the opportunity to demonstrate their knowledge and skills at their current workplace, most would leave the workplace anyway. Conclusion: Considering the results obtained in the research, it can be noted that for nurses and technicians in the Republic of Croatia, good working conditions and job security are more important motivational factors than salary, which was expected to be the most important factor. According to the research results, it can be noted that a higher level of education does not mean greater motivation.