Background: Professional burnout is a mental health problem, which is classically defined as a long-term response to work-related stress, and is associated with reduced personal achievement or inefectiveness, depersonalization or cynicism, as well as emotional exhaustion at the workplace. It can be exacerbated by several factors, including heavy workloads, unhealthy work environments and lack of organizational support. Aim: The objective of this research is to examine the level of stress and factors related to the stress of nurses and technicians at the surgical workplaces in the Dubrava Clinical Hospital. Subjects and methods: A total of 120 respondents participated in the research, of which 40 were from surgical departments, 40 from the surgical theater and 40 from the unified emergency hospital admission. The respondents were female in 67.5%, and male in 32.5%. The average age of the respondents was from 26 to 35 years old in all three observed groups (45%), and the arithmetic mean for age is 34. 5. A statistically significant difference in the impact of stress was observed in nurses/technicians of surgical departments, especially in administrative tasks. The difference in the impact of stress according to gender was not statistically significant. Results: A total of 120 respondents participated in the research, of which 40 were from surgical departments, 40 from the surgical theater and 40 from the unified emergency hospital admission. The respondents were female in 67.5%, and male in 32.5%. The average age of the respondents was from 26 to 35 years old (45%). The value of the Chi-square test is p<0.05, and a statistically significant difference was observed regarding the level of stress among subjects from all three examined groups. A significant level of stress was observed in respondents aged 26 to 35 years. Conclusion: The obtained results confirmed that the proposed stress-related factors cause a significant level of stress in all the examined groups. Given that most respondents were female in all the examined groups, it can be concluded that a higher level of stress was recorded in females. Respondents from all three groups have the same level of stress according to the examined factors, regardless of the surgical workplace. The factors that most influence the appearance of stress in surgical nurses and technicians are administrative tasks.