Background Knowledge and education about palliative care and positive attitudes of nurses and medical technicians towards the implementation of palliative care are basic prerequisites for improving palliative care and providing the best possible conditions for improving the quality of life and dying worthy of a human being. Aim The aim of this research is to identify the level of knowledge and identify changes in attitudes about palliative care in accordance with the level of education. Methods A quantitative cross-sectional study was conducted on 130 subjects. The study compared the attitudes and knowledge about palliative care of 4th grade medical school students and the 5th grade of secondary medical school majoring in general care nurse/general care medical technician to identify changes in attitudes and knowledge about palliative care during schooling. Attitudes and knowledge about palliative care of students in the final grades of medical school in the field of general care nurse/general care medical technician were compared with nurses/medical technicians of different levels of education. Results Positive attitudes of students and nurses/technicians were established, as well as relatively low knowledge of both students and nurses/technicians about palliative care. Nurses have more knowledge than high school students. Conclusion Although the curriculum covers teaching content from palliative care throughout all three years of vocational education, the amount of teaching content should be increased to enable further development of palliative care.