Introduction: By definition, pain is an uncomfortable sense and emotional experience of an individual. The wrong pain management can lead to serious consequences and can very often result in unnecessary suffering for the patient. Because of its clinical importance, pain is defined as the fifth vital sign. The right knowledge and attitude coming from medical nurses/technicians, who are valuable participants in discovering and treating pain, can contribute to better pain management and can improve patient’s life quality. Goal: The goal of this research is to identify and analyze the attitude and degree of knowledge that medical nurses/technicians possess in treating pain of cancer patients at the Oncology Clinic in Zagreb and to determine the correlation between degree of knowledge and years of experience to knowledge and stand towards pain management. Methods: The structure of research is quantitative descriptive research. The research includes medical nurses/technicians at the Oncology Clinic, University Hospital Clinic Zagreb, with different knowledge and years of experience. The instrument of research is a questionnaire from B.R. Ferrell and M. McCaffery “Knowledge and attitudes survey regarding pain” which consists of 39 questions and has for the purposes of this research been reduced to 24 questions and translated to Croatian. Results: According to the results obtained from the sample group of medical nurses and technicians at the Oncology Clinic, University Hospital Clinic in Zagreb, the medical staff possess mostly low to average knowledge in treating pain in cancer patients. 47,7% of respondents have accomplished results higher than average (14+ correct answers). Results show there is a statistically significant difference (p <0.05) among nurses/technicians with higher level of education and several years of experience in achieving better results. Conclusion: According to the obtained results, we can conclude that the level of education and years of experience affect the attitude and knowledge on pain treatment of medical nurses/techinicans. However, looking at the overall knowledge of nurses, we can conclude that education of health professionals is needed to contribute to better knowledge and attitudes in the treatment of oncological pain.