Introduction: Organ transplantation is in some cases the only treatment option. With the advancement of medicine, organ transplantation is nowadays more accepted and successful way of treatment. Organ transplantation largely depends on organ donors. Therefore, great importance should be given to education, not only of general population, but also of healthcare professionals. In the Republic of Croatia, a donor is considered to be any person who has not explicitly expressed otherwise during his lifetime. Croatia is a country that is a member of the Eurotransplant organization and that with its National programme belongs to the leading countries of the world. In transplant medicine, a nurse also plays a big role. Aim: The aim of this study was to examine the attitudes of employed nurses and nursing students on organ donation and to examine and analyze whether there are major differences in their attitudes. Methods: For the purpose of this research was used questionnare made in Google Forms and was shared via a social network (Facebook). The survey contained 21 question. The data was processed using the programming language R (version 4.0.5, Ross Ihaka and Robert Gentlemen, Auckland, New Zeland). In the survey participated 763 respondents, but due to inadequate filling of survey 750 were included. Results: The results show that working nurses have more confidence in doctors, that they are more willing to donate organs of their loved ones, that they are more willing to donate during their lifetime, and that they do not think it would change the cours of their life and they are also more open to accepting the donated organ. Both students and working nurses support registration for organ donation, however working nurses also suport automatic registration in a greater extent. The results also showed that respondent's attitudes were more positive with the higher level of their education. Conclusion: The general population and the healthcare professionals are informed about organ donation and transplantation but not in sufficient quantity. Additional education and promotion should ensure more positive attitudes of population, whether general population of healthcare professionals.