According to previous research, personality traits have been shown to be the most consistent and strongest factor in subjective well-being. This research included the cognitive part of subjective well-being, a life satisfaction. Life satisfaction is defined as a report on how an individual evaluates and values his or her entire life. Furthermore, the construct of physical activity proved to be extremely important in assessing the quality of one's own life, both in terms of physical and mental health. Therefore, this research aimed to examine whether personality traits and physical activity predict life satisfaction. The study involved 476 participants aged 16 to 71, with different education, employment and level of physical activity. In order to collect data, three questionnaires were used with previous questions on sociodemographic data. The measuring instruments used are the abbreviated version of the International Physical Activity Questionnaire (IPAQ-SF, 2002), the Life Satisfaction Scale (Penezić, 1996) and the Croatian version of the IPIP-50 personality questionnaire (Mlačić and Goldberg, 2007). The results of the two-step hierarchical regression analysis showed a significant contribution of personality traits which are emotional stability, extraversion, and intellect, while physical activity did not significantly predict life satisfaction. A significant difference in life satisfaction assessment was also found between vigorous active participants and inactive ones, with vigorous active participants estimating a higher level of life satisfaction compared to inactive participants.